Difference between revisions of "Part:BBa K2586007"
Dschannek96 (Talk | contribs) |
|||
| Line 7: | Line 7: | ||
<i>aroA</i> encodes the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, which is the main target of the herbicide glyphosate and is strongly inhibited through glyphosate application. An amplification of <i>aroE/aroA</i> allows for glyphosate resistance (1), this is used in multiple GM plants to increase the yield and improve farming applications. | <i>aroA</i> encodes the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, which is the main target of the herbicide glyphosate and is strongly inhibited through glyphosate application. An amplification of <i>aroE/aroA</i> allows for glyphosate resistance (1), this is used in multiple GM plants to increase the yield and improve farming applications. | ||
If exposed to glyphosate, <i>E.coli</i> mutants will make changes in the original sequence of <i>aroA</i> to avoid the lethal effects of the substance. | If exposed to glyphosate, <i>E.coli</i> mutants will make changes in the original sequence of <i>aroA</i> to avoid the lethal effects of the substance. | ||
| + | <style type="text/css"> | ||
| + | .tg {border-collapse:collapse;border-spacing:0;} | ||
| + | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:0px 20px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:black;} | ||
| + | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:0px 20px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:black;} | ||
| + | .tg .tg-4ovm{font-weight:bold;font-size:13px;border-color:#000000;text-align:left;vertical-align:top} | ||
| + | .tg .tg-9mbj{font-size:13px;border-color:#000000;text-align:left;vertical-align:top} | ||
| + | </style> | ||
| + | <table class="tg"> | ||
| + | <tr> | ||
| + | <td class="tg-4ovm">Locus</td> | ||
| + | <td class="tg-9mbj">BSU29750</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class="tg-4ovm">Isoelectric point</td> | ||
| + | <td class="tg-9mbj">5.34<br></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class="tg-4ovm">Molecular weight</td> | ||
| + | <td class="tg-9mbj">39.38 kDa</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class="tg-4ovm">Protein length</td> | ||
| + | <td class="tg-9mbj">358 aa</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class="tg-4ovm">Gene length</td> | ||
| + | <td class="tg-9mbj">1074 bp<br></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class="tg-4ovm">Function</td> | ||
| + | <td class="tg-9mbj">biosynthesis of aromatic amino acids</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class="tg-4ovm">Product</td> | ||
| + | <td class="tg-9mbj">3-deoxy-D-arabino-heptulosonate 7-phosphate synthase/<br>chorismate mutase-isozyme 3<br></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class="tg-4ovm">Essential</td> | ||
| + | <td class="tg-9mbj">no</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class="tg-4ovm">Synonyms</td> | ||
| + | <td class="tg-9mbj">aroG</td> | ||
| + | </tr> | ||
| + | </table> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 15:01, 23 September 2018
aroA: 3-phosphoshikimate 1-carboxyvinyltransferase
This part encodes for the 3-phosphoshikimate 1-carboxyvinyltransferase.
The encoded enzyme is needed for the biosynthesis of essential aromatic amino acids in the shikimate pathway. Three essential aromatic amino acids are products of the pathway: Trp, Phe and Tyr. aroA is expressed in the E.coli wildtype.
aroA encodes the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, which is the main target of the herbicide glyphosate and is strongly inhibited through glyphosate application. An amplification of aroE/aroA allows for glyphosate resistance (1), this is used in multiple GM plants to increase the yield and improve farming applications.
If exposed to glyphosate, E.coli mutants will make changes in the original sequence of aroA to avoid the lethal effects of the substance.
<style type="text/css">
.tg {border-collapse:collapse;border-spacing:0;}
.tg td{font-family:Arial, sans-serif;font-size:14px;padding:0px 20px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:black;}
.tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:0px 20px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:black;}
.tg .tg-4ovm{font-weight:bold;font-size:13px;border-color:#000000;text-align:left;vertical-align:top}
.tg .tg-9mbj{font-size:13px;border-color:#000000;text-align:left;vertical-align:top}
</style>
| Locus | BSU29750 |
| Isoelectric point | 5.34 |
| Molecular weight | 39.38 kDa |
| Protein length | 358 aa |
| Gene length | 1074 bp |
| Function | biosynthesis of aromatic amino acids |
| Product | 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase/ chorismate mutase-isozyme 3 |
| Essential | no |
| Synonyms | aroG |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 346
Illegal AgeI site found at 787 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1021