Difference between revisions of "Part:BBa K2586007"

Revision as of 06:56, 13 September 2018

aroA: 3-phosphoshikimate 1-carboxyvinyltransferase

This part encodes for the 3-phosphoshikimate 1-carboxyvinyltransferase. The encoded enzyme is needed for the biosynthesis of essential aromatic amino acids in the shikimate pathway. Three essential aromatic amino acids are products of the pathway: Trp, Phe and Tyr. aroA is expressed in the E.coli wildtype.
aroA encodes the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, which is the main target of the herbicide glyphosate and is strongly inhibited through glyphosate application. An amplification of aroE/aroA allows for glyphosate resistance (1), this is used in multiple GM plants to increase the yield and improve farming applications. If exposed to glyphosate, E.coli mutants will make changes in the original sequence of aroA to avoid the lethal effects of the substance.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 346
Illegal AgeI site found at 787
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1021

@@ Line 5: / Line 5: @@
 This part encodes for the 3-phosphoshikimate 1-carboxyvinyltransferase.
 The encoded enzyme is needed for the biosynthesis of essential aromatic amino acids in the shikimate pathway. Three essential aromatic amino acids are products of the pathway: Trp, Phe and Tyr. <i>aroA</i> is expressed in the <i> E.coli</i> wildtype. <br/>
-<i>aroA</i> encodes the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, which is the main target of the herbicide glyphosate and is strongly inhibited through glyphosate application. An amplification of aroE allows for glyphosate resistance (1), this is used in multiple GM plants to increase the yield and improve farming applications.
+<i>aroA</i> encodes the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, which is the main target of the herbicide glyphosate and is strongly inhibited through glyphosate application. An amplification of <i>aroE/aroA</i> allows for glyphosate resistance (1), this is used in multiple GM plants to increase the yield and improve farming applications.
 If exposed to glyphosate, <i>E.coli</i> mutants will make changes in the original sequence of <i>aroA</i> to avoid the lethal effects of the substance.