Difference between revisions of "Part:BBa I715052"
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<partinfo>BBa_I715052 short</partinfo> | <partinfo>BBa_I715052 short</partinfo> | ||
| − | + | The <i>E. coli trp</i> operon is responsible for synthesis of tryptophan when its cellular concentration is low. This part was amplified using PCR from the chromosome. It contains a predicted RBS, the leader peptide, and the regulatory sequence with the anti-terminator and terminator. | |
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | In the presence of tryptophan, any sequences downstream will not be transcribed. In the absence of tryptophan they will be transcribed. This part can therefore serve as an "off" switch for turning off the expression of any downstream genes. | ||
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Latest revision as of 17:38, 23 March 2008
Trp Leader Peptide and anti-terminator/terminator
The E. coli trp operon is responsible for synthesis of tryptophan when its cellular concentration is low. This part was amplified using PCR from the chromosome. It contains a predicted RBS, the leader peptide, and the regulatory sequence with the anti-terminator and terminator.
Usage and Biology
In the presence of tryptophan, any sequences downstream will not be transcribed. In the absence of tryptophan they will be transcribed. This part can therefore serve as an "off" switch for turning off the expression of any downstream genes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]