Difference between revisions of "Part:BBa I757011"
| Line 10: | Line 10: | ||
* Synthetic DNA by GeneArt | * Synthetic DNA by GeneArt | ||
* Part in pGA4 vector (AmpR, ColEI ori) | * Part in pGA4 vector (AmpR, ColEI ori) | ||
| + | * Part contains amino acids 26-199 of TEM-116 lactamase according to the numbering of Ambler, or amino acids 24-197 according to consecutive numbering with signal sequence. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 08:31, 13 July 2010
bla_frag1(aa 26-199) Fusion Part
- first fragment of the split enzyme TEM beta-lactamase
- purpose: split lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains
- works with second half of split lactamase (part BBa_I757012)
- this fragment contains stabilizing mutations
- NgoMIV / AgeI protein fusion part
- iGEM Team Freiburg 2007
- Synthetic DNA by GeneArt
- Part in pGA4 vector (AmpR, ColEI ori)
- Part contains amino acids 26-199 of TEM-116 lactamase according to the numbering of Ambler, or amino acids 24-197 according to consecutive numbering with signal sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 535 - 1000COMPATIBLE WITH RFC[1000]