Difference between revisions of "Part:BBa J100455"
 
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<partinfo>BBa_J100455 short</partinfo>
 
<partinfo>BBa_J100455 short</partinfo>
  
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The -17 to -12 bases have been removed from the T7 consensus sequence and replaced by the Gal4 binding site. The 2 bp deletion in the Gal4 binding site should prevent binding of the Gal4 protein. To be used as a negative control in testing part [https://parts.igem.org/Part:BBa_J100425 J100425]
+
The -17 to -12 bases have been removed from the T7 consensus sequence and replaced by the Gal4 binding site. The 2 bp deletion in the Gal4 binding site has been introduced using PCR primers to rprevent binding of the Gal4 protein. To be used as a negative control in testing part [https://parts.igem.org/Part:BBa_J100425 J100425]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:15, 9 September 2018


Gal4/GFP/Streptavidin Fusion Protein with Mutated Gal4 Binding Site in T7 Promoter

The -17 to -12 bases have been removed from the T7 consensus sequence and replaced by the Gal4 binding site. The 2 bp deletion in the Gal4 binding site has been introduced using PCR primers to rprevent binding of the Gal4 protein. To be used as a negative control in testing part J100425

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5
    Illegal suffix found in sequence at 1871
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5
    Illegal NheI site found at 1816
    Illegal SpeI site found at 1872
    Illegal PstI site found at 1886
    Illegal NotI site found at 11
    Illegal NotI site found at 1879
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5
    Illegal XhoI site found at 339
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5
    Illegal suffix found in sequence at 1872
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5
    Illegal XbaI site found at 20
    Illegal SpeI site found at 1872
    Illegal PstI site found at 1886
    Illegal NgoMIV site found at 1379
    Illegal NgoMIV site found at 1772
    Illegal AgeI site found at 1505
  • 1000
    COMPATIBLE WITH RFC[1000]