Difference between revisions of "Part:BBa K2810007:Design"
Ryancoates (Talk | contribs) (Created page with " __NOTOC__ <partinfo>BBa_K2810007 short</partinfo> <partinfo>BBa_K2810007 SequenceAndFeatures</partinfo> ===Design Notes=== Cloned into golden gate compatible pSB1C3 (P1050...") |
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The part is GoldenGate compatible and so has 5' and 3' sticky ends of 4bases to enable the part to ligate into pGBA2. It also has short 3 nucleotide sequences at each end, that when ligated into the pGBA2 plasmid, creates a recognition site for the Type II S restriction enzyme, BsaI. | |
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===Source=== | ===Source=== | ||
| − | + | Myzus persicae genomic sequence | |
===References=== | ===References=== | ||
Latest revision as of 11:01, 31 August 2018
siRNA from 5' region of Myzus persicae SP3 secreted protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is GoldenGate compatible and so has 5' and 3' sticky ends of 4bases to enable the part to ligate into pGBA2. It also has short 3 nucleotide sequences at each end, that when ligated into the pGBA2 plasmid, creates a recognition site for the Type II S restriction enzyme, BsaI.
Source
Myzus persicae genomic sequence