Difference between revisions of "Part:BBa K2615003:Design"

 
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===References===
 
===References===
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[http://science.sciencemag.org/content/329/5997/1355 Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence- and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997):1355-1358.]
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Revision as of 13:42, 11 September 2018


Csy4-WT, the No.1 member of Csy4 family.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 377
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 93


Design Notes

experiments


Source

1.Sternberg S H, Haurwitz R E, Doudna J A. Mechanism of substrate selection by a highly specific CRISPR endoribonuclease[J]. Rna-a Publication of the Rna Society, 2012, 18(4):661-72. 2.Haurwitz R E, Sternberg S H, Doudna J A. Csy4 relies on an unusual catalytic dyad to position and cleave CRISPR RNA[J]. Embo Journal, 2014, 31(12):2824-2832. 3. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence- and Structure-Specific RNA Processing by a CRISPR Endonuclease[J]. Science, 2010, 329(5997):1355-1358. 4. Cady K C, O'Toole G A. Non-identity-mediated CRISPR-bacteriophage interaction mediated via the Csy and Cas3 proteins[J]. Journal of Bacteriology, 2011, 193(14):3433-3445.

References

[http://science.sciencemag.org/content/329/5997/1355 Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence- and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997):1355-1358.]