Difference between revisions of "Part:BBa K2624005:Design"
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===Design Notes=== | ===Design Notes=== | ||
We checked the exact transcription start site of the hulc gene and replaced the sequence after it with the sequence that we designed so that, instead of the lncRNA, it would express the pri-miRNA analogue. | We checked the exact transcription start site of the hulc gene and replaced the sequence after it with the sequence that we designed so that, instead of the lncRNA, it would express the pri-miRNA analogue. | ||
| − | Then of course we added a SV40 poly(A) sequence that signals the end of a transcriptional unit. | + | Then of course we added a SV40 poly(A) sequence that signals the end of a transcriptional unit. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 09:39, 17 August 2018
hulc-pri-miRNA(MAP4K4)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 130
Design Notes
We checked the exact transcription start site of the hulc gene and replaced the sequence after it with the sequence that we designed so that, instead of the lncRNA, it would express the pri-miRNA analogue. Then of course we added a SV40 poly(A) sequence that signals the end of a transcriptional unit.
Source
Hulc promoter sequence comes from Swissregulon Database. The pri-miRNA analogue part is designed by us.
References
[1]Wang J, Liu X, Wu H, et al. CREB up-regulates long non-coding RNA, HULC expression through interaction with microRNA-372 in liver cancer[J]. Nucleic acids research, 2010, 38(16): 5366-5383. [2]Cheng H, Zhang Y, Wang H, et al. Regulation of MAP4K4 gene expression by RNA interference through an engineered theophylline-dependent hepatitis delta virus ribozyme switch[J]. Molecular BioSystems, 2016, 12(11): 3370-3376.