Difference between revisions of "Part:BBa K2624005"

Revision as of 09:31, 17 August 2018

hulc-pri-miRNA(MAP4K4)

This composite consists of two parts. One is the core promoter of the gene highly up-regulated in liver cancer (HULC), the other designed to encode a primary microRNA analogue targeting MAP4K4. This expression unit is up-regulated in hepatocellular carcinoma and liver cancer cell lines at transcription level, hence constitutive knock-down of MAP4K4 if such cells are transfected with.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 130

Revision as of 09:02, 17 August 2018 (view source) Registry (Talk \| contribs)		Revision as of 09:31, 17 August 2018 (view source) Zhoushuyao (Talk \| contribs) Newer edit →
Line 3:		Line 3:
	<partinfo>BBa_K2624005 short</partinfo>		<partinfo>BBa_K2624005 short</partinfo>

−	This composite consists of two parts. One is the core promoter of the gene highly up-regulated in liver cancer (HULC), the other designed to encode a ~~primiry~~ microRNA analogue targeting MAP4K4. This expression unit is up-regulated in hepatocellular carcinoma and liver cancer cell lines at transcription level, hence constitutive knock-down of MAP4K4 if such cells are transfected with.	+	This composite consists of two parts. One is the core promoter of the gene highly up-regulated in liver cancer (HULC), the other designed to encode a primary microRNA analogue targeting MAP4K4. This expression unit is up-regulated in hepatocellular carcinoma and liver cancer cell lines at transcription level, hence constitutive knock-down of MAP4K4 if such cells are transfected with.