Difference between revisions of "Part:BBa K2719001:Experience"
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===Applications of BBa_K2719001=== | ===Applications of BBa_K2719001=== | ||
| + | <p>To confirm the presence of Tenascin, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in Escherichia coli DH5a. (Figure 2)</p> | ||
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| + | <p>To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of GST. (Figure 4 and 3)</p> | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 10:51, 17 October 2018
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how you used this part and how it worked out.
Applications of BBa_K2719001
To confirm the presence of Tenascin, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in Escherichia coli DH5a. (Figure 2)
To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of GST. (Figure 4 and 3)
User Reviews
UNIQ48d30dc69543e366-partinfo-00000000-QINU UNIQ48d30dc69543e366-partinfo-00000001-QINU