Difference between revisions of "Part:pSB1A7"

Latest revision as of 17:32, 12 July 2018

Transcriptionally insulated high copy BioBrick plasmid

pSB1A7 was constructed by adding double terminators (BBa_B0015) before the BioBrick prefix and after the BioBrick suffix in pSB1A3. The added terminators block read-through transcription coming from the vector backbone into the BioBrick MCS.

pSB1A7 was promoted from BBa_J31009 by Randy on 11/16/2006.

pSB1A8 is a newer, modified version of pSB1A7 with the BsaI site in the ampR gene removed (J119043).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2411
Illegal XbaI site found at 2426
Illegal SpeI site found at 1
Illegal PstI site found at 15
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2411
Illegal SpeI site found at 1
Illegal PstI site found at 15
Illegal NotI site found at 8
Illegal NotI site found at 2417
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2411
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 2411
Plasmid lacks a suffix.
Illegal XbaI site found at 2426
Illegal SpeI site found at 1
Illegal PstI site found at 15
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 1312

@@ Line 6: / Line 6: @@
 pSB1A7 was promoted from BBa_J31009 by Randy on 11/16/2006.
-pSB1A8 is a modified version of pSB1A7 with the BsaI site in the ampR gene removed [https://parts.igem.org/Part:BBa_J119043 (J119043)].
+pSB1A8 is a newer, modified version of pSB1A7 with the BsaI site in the ampR gene removed [https://parts.igem.org/Part:BBa_J119043 (J119043)].
 <!-- Add more about the biology of this part here