Difference between revisions of "Part:BBa J100416"
Tatravieso (Talk | contribs) |
Tatravieso (Talk | contribs) |
||
Line 3: | Line 3: | ||
<partinfo>BBa_J100416 short</partinfo> | <partinfo>BBa_J100416 short</partinfo> | ||
− | T7 consensus sequence [https://parts.igem.org/Part:BBa_R0085 | + | T7 consensus sequence [https://parts.igem.org/Part:BBa_R0085 (R0085)] inserted into pClone Red <a href="https://parts.igem.org/Part:BBa_J119137">(J119137)</a>, encoding for RFP. Used as a control to test against experimental promoters that had Gal4 binding sites upstream, downstream, and within this promoter sequence. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 14:10, 26 June 2018
T7 Consensus Promoter in pClone Red
T7 consensus sequence (R0085) inserted into pClone Red <a href="https://parts.igem.org/Part:BBa_J119137">(J119137)</a>, encoding for RFP. Used as a control to test against experimental promoters that had Gal4 binding sites upstream, downstream, and within this promoter sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1437
Illegal AgeI site found at 1549 - 1000COMPATIBLE WITH RFC[1000]