Difference between revisions of "Part:BBa J100416"

Revision as of 14:08, 26 June 2018

T7 Consensus Promoter in pClone Red

T7 consensus sequence <a href="https://parts.igem.org/Part:BBa_R0085">(R0085)</a> inserted into pClone Red <a href="https://parts.igem.org/Part:BBa_J119137">(J119137)</a>, encoding for RFP. Used as a control to test against experimental promoters that had Gal4 binding sites upstream, downstream, and within this promoter sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1437
Illegal AgeI site found at 1549
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_J100416 short</partinfo>
-T7 consensus sequence <a href="https://parts.igem.org/Part:BBa_R0085">(R0085)</a> inserted into pClone Red <a href="https://parts.igem.org/Part:BBa_J119137">(J119137)</a>, encoding for RFP. Used as a control to test against experimental promoters that had Gal4 binding sites upstream, downstream, and within this promoter sequence.
+T7 consensus sequence <a href="https://parts.igem.org/Part:BBa_R0085">(R0085)</a>  inserted into pClone Red <a href="https://parts.igem.org/Part:BBa_J119137">(J119137)</a>, encoding for RFP. Used as a control to test against experimental promoters that had Gal4 binding sites upstream, downstream, and within this promoter sequence.
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