Difference between revisions of "Part:BBa K2876002:Design"
(→Design Notes) |
|||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | codon optimized for E. coli | + | codon optimized for E. coli, with the additional constraint of codon optimization to remove restriction sites allowing for pSB1C3 compatibility. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 06:58, 17 October 2018
RNAPolIII alpha subunit
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 8
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 480
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
codon optimized for E. coli, with the additional constraint of codon optimization to remove restriction sites allowing for pSB1C3 compatibility.
Source
Dove, S. L., Joung, J. K., & Hochschild, A. (1997). Activation of prokaryotic transcription through arbitrary protein- protein contacts. Nature. http://doi.org/10.1038/386627a0
References
Dove, S. L., Joung, J. K., & Hochschild, A. (1997). Activation of prokaryotic transcription through arbitrary protein- protein contacts. Nature. http://doi.org/10.1038/386627a0