Difference between revisions of "Part:BBa K2876001"

 
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<partinfo>BBa_K2876001 short</partinfo>
 
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This is a Lambda CL protein that can be linked to a bait protein to function as part of a two hybrid system in E. coli. This gene was sourced from an Addgene plasmid (https://www.addgene.org/53730/), and was deposited by Anna Hochschild from her paper Activation of Prokaryotic Transcription Through Arbitrary Protein-Protein Contacts (Dove, Joung, Hochschild, Nature 1997)
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This is a Lambda CL protein that can be linked to a bait protein to function as part of a two hybrid system in E. coli  
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===Usage and Biology===
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The Lambda CL protein is normally a repressor, but can be fused to a protein as part of a prokaryotic two hybrid transcription system.
  
 
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Revision as of 06:28, 17 October 2018


Lambda CL DNA inhibitor This is a Lambda CL protein that can be linked to a bait protein to function as part of a two hybrid system in E. coli. This gene was sourced from an Addgene plasmid (https://www.addgene.org/53730/), and was deposited by Anna Hochschild from her paper Activation of Prokaryotic Transcription Through Arbitrary Protein-Protein Contacts (Dove, Joung, Hochschild, Nature 1997)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]