Difference between revisions of "Part:BBa J100413"
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<partinfo>BBa_J100413 short</partinfo>
 
<partinfo>BBa_J100413 short</partinfo>
  
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Gal4 binding site inserted upstream of a T7 promoter consensus sequence that encodes for RFP when inserted in pClone. When present the Gal4 protein will bind to this site.  
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Gal4 binding site inserted upstream of a T7 promoter consensus sequence [https://parts.igem.org/Part:BBa_J100402 (J100402] that will produce RFP when inserted into pClone [https://parts.igem.org/Part:BBa_J119137 (J119137)]. When present the Gal4 protein will bind to this site.  
  
 
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Revision as of 14:19, 26 June 2018


T7 Promoter with Upstream Gal4 Binding Site in pClone Red

Gal4 binding site inserted upstream of a T7 promoter consensus sequence (J100402 that will produce RFP when inserted into pClone (J119137). When present the Gal4 protein will bind to this site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1454
    Illegal AgeI site found at 1566
  • 1000
    COMPATIBLE WITH RFC[1000]