Difference between revisions of "Part:BBa M50420:Design"
Line 16: Line 16:
 
Promoter: BBa_K914003
 
Promoter: BBa_K914003
 
https://parts.igem.org/wiki/index.php?title=Part:BBa_K914003
 
https://parts.igem.org/wiki/index.php?title=Part:BBa_K914003
−
 
−
<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K914003
 
−
">BBa_K914003</a>
 
  
 
RBS: BBa_B0034
 
RBS: BBa_B0034
 +
https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034
  
 
Leader Sequence: BBa_J32015
 
Leader Sequence: BBa_J32015
 +
https://parts.igem.org/Part:BBa_J32015
  
 
Gene: BBa_K332011 codon optimized for E. coli
 
Gene: BBa_K332011 codon optimized for E. coli
 +
https://parts.igem.org/Part:BBa_K332011
  
 
6x-His Tag: BBa_K1223006
 
6x-His Tag: BBa_K1223006
 +
https://parts.igem.org/Part:BBa_K1223006
  
 
Terminator: BBa_B0012
 
Terminator: BBa_B0012
−
 
+
https://parts.igem.org/Part:BBa_B0012
  
 
===References===
 
===References===

Revision as of 04:25, 12 June 2018


Mosquito Larvae Insecticide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 188
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We codon optimized the Cry11Aa amino acid sequence for E. coli. All of our parts were sourced from existing iGEM parts which are listed below. We did not make any other modifications from the source sequences.


Source

The source is previously established parts and the protein from uniprot cry11Aa.

Promoter: BBa_K914003 https://parts.igem.org/wiki/index.php?title=Part:BBa_K914003

RBS: BBa_B0034 https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034

Leader Sequence: BBa_J32015 https://parts.igem.org/Part:BBa_J32015

Gene: BBa_K332011 codon optimized for E. coli https://parts.igem.org/Part:BBa_K332011

6x-His Tag: BBa_K1223006 https://parts.igem.org/Part:BBa_K1223006

Terminator: BBa_B0012 https://parts.igem.org/Part:BBa_B0012

References