Difference between revisions of "Part:BBa I13522"
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===Usage and Biology===
 
===Usage and Biology===
 
GFP only control
 
GFP only control
 +
 +
The version shipped with the 2008 iGEM distribution does not appear to function. Unclear why. [[User:Jkm|Jkm]] 18:40, 22 August 2008 (UTC)
 
<br><br>
 
<br><br>
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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==Characterization of [[Chassis/Cell-Free_Systems|Cell-Free Chassis]]==
 
==Characterization of [[Chassis/Cell-Free_Systems|Cell-Free Chassis]]==
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This part was used to characterize the cell-free chassis. Details of characterization can be found on the [[Chassis/Cell-Free Systems/Commercial E.coli S30|cell-free characterization]] page. Other interesting isothermal properties of this construct are available at the [http://parts.mit.edu/igem07/index.php/Imperial/Cell_by_Date/Testing Cell By Date project page] of Imperial 2007 wiki.
+
This part was used to characterize the cell-free chassis. Details of characterization can be found on the [[Chassis/Cell-Free Systems/Commercial E.coli S30|cell-free characterization]] page. Other interesting isothermal properties of this construct are available at the [http://2007.igem.org/Imperial/Cell_by_Date/Testing Cell By Date project page] of Imperial 2007 wiki.
 
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{|border=1 width="90%" align="center"
 
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Revision as of 18:40, 22 August 2008

pTet GFP

Untagged GFP behind a constitutive promoter.

Usage and Biology

GFP only control

The version shipped with the 2008 iGEM distribution does not appear to function. Unclear why. Jkm 18:40, 22 August 2008 (UTC)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


Pictures

  • BBa_I13522 visualized under non-UV lightbox

  • BBa_I13522 visualized under 254nm wavelength UV lightbox

Characterization of Cell-Free Chassis

This part was used to characterize the cell-free chassis. Details of characterization can be found on the cell-free characterization page. Other interesting isothermal properties of this construct are available at the [http://2007.igem.org/Imperial/Cell_by_Date/Testing Cell By Date project page] of Imperial 2007 wiki.

Parameter Value and Description
Optimal Temperature Expression increases with temperature from 4°C to 30°C
Optimal DNA Concentration Works optimally with 2µg of DNA in 60 µl of cell-extract
Peak Expression Time Peak rate of output occurs after approximately 85 minutes
Expression Lifespan Expression levels off after 2 hours
Expression Capacity Can synthesize over 200pmols of GFPmut3b in 6 hours