Difference between revisions of "Part:BBa M50437:Design"
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===Source=== | ===Source=== | ||
| − | T5 promoter: BBa_M50075 | + | T5 promoter: BBa_M50075 <BR> |
| − | Strong RBS: BBa_M50080 | + | Strong RBS: BBa_M50080 <BR> |
| − | entC: BBa_M50436 | + | entC: BBa_M50436 <BR> |
| − | ahpC: BBa_M50435 | + | ahpC: BBa_M50435 <BR> |
| − | T7 terminator: BBa_M50080 | + | T7 terminator: BBa_M50080 <BR> |
Revision as of 23:10, 11 June 2018
2,3-DHB Biosynthesis Construct, with entC and ahpC
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 234
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 234
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 234
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 234
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 234
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Our plasmid contained two distinct coding sequences, separately delineated with ribosome-binding sites.
Source
T5 promoter: BBa_M50075
Strong RBS: BBa_M50080
entC: BBa_M50436
ahpC: BBa_M50435
T7 terminator: BBa_M50080