Difference between revisions of "Part:BBa M50437:Design"
Line 11: Line 11:
 
===Source===
 
===Source===
  
−
T5 promoter: BBa_K784005
+
T5 promoter: BBa_M50075
 +
Strong RBS: BBa_M50080
 +
entC: BBa_M50436
 +
ahpC: BBa_M50435
 +
T7 terminator: BBa_M50080

Revision as of 23:09, 11 June 2018

2,3-DHB Biosynthesis Construct, with entC and ahpC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 234
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 234
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 234
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our plasmid contained two distinct coding sequences, separately delineated with ribosome-binding sites.

Source

T5 promoter: BBa_M50075 Strong RBS: BBa_M50080 entC: BBa_M50436 ahpC: BBa_M50435 T7 terminator: BBa_M50080