Difference between revisions of "Part:BBa M50439:Experience"

 
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===Applications of BBa_M50439===
 
===Applications of BBa_M50439===
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This construct can successfully be used to measure intracellular pH given by a BRET ratio in S. cerevisiae. Due to the well-characterized pH-sensitivity of Rluc8 (luciferase), we are confident that BRET readout from cpVenus (fluorophore) is indicative of intracellular pH because it was excited through Rluc8 activity.
  
 
===User Reviews===
 
===User Reviews===
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<I>Username</I>
 
<I>Username</I>
 
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Enter the review inofrmation here.
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Enter the review information here.
 
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<!-- DON'T DELETE --><partinfo>BBa_M50439 EndReviews</partinfo>
 
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===This plasmid was used in Bba_M50440 and the user experience is better outlined there.===
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===Stanford Location===
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Plasmid name: pHlash<BR>
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DNA2.0 Gene #: custom version of <BR>
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Organism: S. cerevisiae <BR>
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Device type: Sensor<BR>
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Glycerol stock barcode: 133021752<BR>
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Box label: BioE44 S18

Revision as of 11:51, 12 June 2018


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_M50439

This construct can successfully be used to measure intracellular pH given by a BRET ratio in S. cerevisiae. Due to the well-characterized pH-sensitivity of Rluc8 (luciferase), we are confident that BRET readout from cpVenus (fluorophore) is indicative of intracellular pH because it was excited through Rluc8 activity.

User Reviews

UNIQ5ac7a89042b26809-partinfo-00000000-QINU UNIQ5ac7a89042b26809-partinfo-00000001-QINU

This plasmid was used in Bba_M50440 and the user experience is better outlined there.

Stanford Location

Plasmid name: pHlash
DNA2.0 Gene #: custom version of
Organism: S. cerevisiae
Device type: Sensor
Glycerol stock barcode: 133021752
Box label: BioE44 S18