Difference between revisions of "Part:BBa I721003"
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'''Description'''<br>
 
'''Description'''<br>
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This part is designed to enable Lead Detection in a cell. When lead enters the cell, it will couple with the Lead Binding protein, forming a dimer. This dimer will then bind to the Lead Promoter, which is also contained within this part, BBa_I1721003.
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This part is designed to enable Lead Detection in a cell. When Lead (Pb +2) enters the cell, it will couple with the Lead Binding protein, forming a dimer. This dimer will then bind to the Lead Promoter, which is also contained within this part, BBa_I721003. BBa_I721003 contains both the Lead Binding Protein sequence and the Lead Promoter sequence, and should form a standalone Lead Detection system.
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This sequence was obtained using PCR from the genome of Ralstonia metallidurans. Biobrick cut sites were added using specific primers during PCR.
  
 
This part is not known to function. "PbrR691/promoter combinations in presence of lead nitrate give no GFP production and no AHL production compared to control. "
 
This part is not known to function. "PbrR691/promoter combinations in presence of lead nitrate give no GFP production and no AHL production compared to control. "
  
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In the diagram below, this part contains three sections: A sequence for a Lead Binding Protein, a non-coding region, and a Lead Promoter.
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This part contains three sections: A sequence for a Lead Binding Protein, a non-coding region, and a Lead Promoter.
  
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[[Image:BBa_1721003_1.jpg|thumb|left|Functional layout of this sequence]]
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[[Image:BBa_1721003_1.jpg|thumb|left|Sequence Layout of I721003]]
  
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[[Image:BBa_1721003_2.jpg|thumb|left|Overall Brown University iGEM 07 Design]]
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[[Image:BBa_1721003_4.jpg|thumb|left|15 planned Lead Promoter and Lead Binding Protein Biobricks]]
  
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[[Image:BBa_1721003_3.jpg|thumb|left|Functional Layout of I721003]]
  
  
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[[Image:BBa_1721003_4.jpg|thumb|left|15 planned Lead Promoter and Lead Binding Protein Biobricks]]
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[[Image:BBa_1721003_2.jpg|thumb|left|Overall Brown University iGEM 07 Design]]
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[[Image:BBa_1721003_3.jpg|thumb|left|15 planned Lead Promoter and Lead Binding Protein Biobricks]]
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Revision as of 23:07, 26 October 2007

Lead Binding Protein + Promoter (version b)

lead binding protein + promoter (part 8)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 479



Part BBa_1721003 is the Lead Binding Protein + Lead Promoter system

Description
This part is designed to enable Lead Detection in a cell. When Lead (Pb +2) enters the cell, it will couple with the Lead Binding protein, forming a dimer. This dimer will then bind to the Lead Promoter, which is also contained within this part, BBa_I721003. BBa_I721003 contains both the Lead Binding Protein sequence and the Lead Promoter sequence, and should form a standalone Lead Detection system.

This sequence was obtained using PCR from the genome of Ralstonia metallidurans. Biobrick cut sites were added using specific primers during PCR.

This part is not known to function. "PbrR691/promoter combinations in presence of lead nitrate give no GFP production and no AHL production compared to control. "

This part contains three sections: A sequence for a Lead Binding Protein, a non-coding region, and a Lead Promoter.

Sequence Layout of I721003
15 planned Lead Promoter and Lead Binding Protein Biobricks
Functional Layout of I721003


Overall Brown University iGEM 07 Design