Difference between revisions of "Part:BBa I721003"
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[[Image:BBa_1721003_1.jpg|thumb|left|Functional layout of this sequence]]
 
[[Image:BBa_1721003_1.jpg|thumb|left|Functional layout of this sequence]]
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[[Image:BBa_1721003_2.jpg|thumb|left|Overall Brown University iGEM 07 Design]]
 
[[Image:BBa_1721003_2.jpg|thumb|left|Overall Brown University iGEM 07 Design]]
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[[Image:BBa_1721003_4.jpg|thumb|left|15 planned Lead Promoter and Lead Binding Protein Biobricks]]
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[[Image:BBa_1721003_3.jpg|thumb|left|15 planned Lead Promoter and Lead Binding Protein Biobricks]]

Revision as of 22:59, 26 October 2007

Lead Binding Protein + Promoter (version b)

lead binding protein + promoter (part 8)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 479



Part BBa_1721003 is the Lead Binding Protein + Lead Promoter system

Description
This part is designed to enable Lead Detection in a cell. When lead enters the cell, it will couple with the Lead Binding protein, forming a dimer. This dimer will then bind to the Lead Promoter, which is also contained within this part, BBa_I1721003.

This part is not known to function. "PbrR691/promoter combinations in presence of lead nitrate give no GFP production and no AHL production compared to control. "

In the diagram below, this part contains three sections: A sequence for a Lead Binding Protein, a non-coding region, and a Lead Promoter.

Functional layout of this sequence
Overall Brown University iGEM 07 Design


15 planned Lead Promoter and Lead Binding Protein Biobricks


15 planned Lead Promoter and Lead Binding Protein Biobricks