Difference between revisions of "Part:BBa I721003"
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'''Description'''<br> | '''Description'''<br> | ||
− | This part is designed to enable Lead Detection in a cell. When lead enters the cell, it will couple with the Lead Binding protein, forming a dimer. This dimer will then bind to the Lead Promoter, which is also contained within this part. | + | This part is designed to enable Lead Detection in a cell. When lead enters the cell, it will couple with the Lead Binding protein, forming a dimer. This dimer will then bind to the Lead Promoter, which is also contained within this part, BBa_I1721003. |
This part is not known to function. "PbrR691/promoter combinations in presence of lead nitrate give no GFP production and no AHL production compared to control. " | This part is not known to function. "PbrR691/promoter combinations in presence of lead nitrate give no GFP production and no AHL production compared to control. " | ||
In the diagram below, this part contains three sections: A sequence for a Lead Binding Protein, a non-coding region, and a Lead Promoter. | In the diagram below, this part contains three sections: A sequence for a Lead Binding Protein, a non-coding region, and a Lead Promoter. | ||
+ | |||
+ | [[Image:BBa_1721003_1.jpg|thumb|left|Functional layout of this sequence]] | ||
+ | [[Image:BBa_1721003_2.jpg|thumb|left|Overall Brown University iGEM 07 Design]] |
Revision as of 22:55, 26 October 2007
Lead Binding Protein + Promoter (version b)
lead binding protein + promoter (part 8)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 479
Part BBa_1721003 is the Lead Binding Protein + Lead Promoter system
Description
This part is designed to enable Lead Detection in a cell. When lead enters the cell, it will couple with the Lead Binding protein, forming a dimer. This dimer will then bind to the Lead Promoter, which is also contained within this part, BBa_I1721003.
This part is not known to function. "PbrR691/promoter combinations in presence of lead nitrate give no GFP production and no AHL production compared to control. "
In the diagram below, this part contains three sections: A sequence for a Lead Binding Protein, a non-coding region, and a Lead Promoter.