Difference between revisions of "Part:BBa I744102"
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<partinfo>BBa_I744102 short</partinfo> | <partinfo>BBa_I744102 short</partinfo> | ||
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| + | Tc -> EYFP | ||
This biobrick is intended to report on the levels of Tetracycline inside a cell. It uses part J23114 (relative strength 256) to drive synthesis of tetR, which determines the sensitivity of the circuit. EYFP is produced for measurement. | This biobrick is intended to report on the levels of Tetracycline inside a cell. It uses part J23114 (relative strength 256) to drive synthesis of tetR, which determines the sensitivity of the circuit. EYFP is produced for measurement. | ||
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| + | For more information go to: [http://parts.mit.edu/igem07/index.php/Rice/Project_A:_Phage_Project#Experimental_System_and_Circuit_Design Rice iGEM 2007 Constructs] | ||
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| + | [[image:Tet_sensor.jpg]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 02:29, 27 October 2007
Tetracycline sensor with medium-strength promoter
Tc -> EYFP
This biobrick is intended to report on the levels of Tetracycline inside a cell. It uses part J23114 (relative strength 256) to drive synthesis of tetR, which determines the sensitivity of the circuit. EYFP is produced for measurement.
For more information go to: [http://parts.mit.edu/igem07/index.php/Rice/Project_A:_Phage_Project#Experimental_System_and_Circuit_Design Rice iGEM 2007 Constructs]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]