Difference between revisions of "Part:BBa K2271016"
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The peroxisomal targeting signal 1 (PTS1) is a protein localization signal which is recognized by the Pex5 receptor protein. Cargo proteins which contain this C-terminal PTS1 tag were imported into the peroxisomes in dependence to Pex5 interaction. The native PTS1 sequence contains three amino acids (SKL motive; Serine-Lysine-Leucin).
 
The peroxisomal targeting signal 1 (PTS1) is a protein localization signal which is recognized by the Pex5 receptor protein. Cargo proteins which contain this C-terminal PTS1 tag were imported into the peroxisomes in dependence to Pex5 interaction. The native PTS1 sequence contains three amino acids (SKL motive; Serine-Lysine-Leucin).
 
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In our project, we designed an artificial PTS1 peptide sequence which is recognized by our modified Pex5 (BBa_K2271067) in order to provide a fully orthogonal peroxisomal protein import machinery in yeast strains deficient for the natural Pex5.
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In our project, we designed an artificial PTS1 peptide sequence which is recognized by our modified Pex5 (BBa_K2271067) in order to provide a fully orthogonal peroxisomal protein import machinery in yeast strains deficient for the natural <i>Pex5</i> gene.
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[[Image:Artico r19lvl2col.png|thumb|center|'''Figure 1:'''PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.]]
 
[[Image:Artico r19lvl2col.png|thumb|center|'''Figure 1:'''PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.]]
 
[[Image:Artico wtpwtpstar.png|thumb|center|'''Figure 2:'''PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.]]
 
[[Image:Artico wtpwtpstar.png|thumb|center|'''Figure 2:'''PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.]]
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<i>Pex5</i> gene.
 
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Revision as of 03:52, 2 November 2017


PTS1 variant P*

The peroxisomal targeting signal 1 (PTS1) is a protein localization signal which is recognized by the Pex5 receptor protein. Cargo proteins which contain this C-terminal PTS1 tag were imported into the peroxisomes in dependence to Pex5 interaction. The native PTS1 sequence contains three amino acids (SKL motive; Serine-Lysine-Leucin).
In our project, we designed an artificial PTS1 peptide sequence which is recognized by our modified Pex5 (BBa_K2271067) in order to provide a fully orthogonal peroxisomal protein import machinery in yeast strains deficient for the natural Pex5 gene.

Figure 1:PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.
Figure 2:PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]