Difference between revisions of "Part:BBa K2423001"

Latest revision as of 03:50, 2 November 2017

CsADH-2946 under the control of a constitutive promoter (BBa_J23106)

This part codes for aldehyde dehydrogenase 2946, which catalyses the conversion of crocetin dialdehyde into crocetin, using NAD+ as a cofactor, in Crocus sativus. It contains a constitutive promoter, as well as a His tag for easy purification. A version of this biobrick containing an inducible promoter is BBa_K2423007.

Usage and Biology

Saffron, a well recognized but expensive spice, has not only uses in terms of cooking but compounds found in saffron have been shown to help with inflammation (1), neurodegenerative diseases (2) and more. Some of those compounds namely zeaxanthin, crocetin dialdehyde, crocetin and crocin are all a part of the same metabolic pathway in the plant specie Crocus Sativus. Not only are these compounds in saffron helpful in terms of their potential medicinal properties, but also the fact that they are very colorful makes them interesting as organic dyes for industrial purposes. These aspects are what drew us at iGEM Uppsala 2017 to work with the pathway from zeaxanthin to crocin in the BioBrick format, but also to integrate the metabolic steps in the pathway from farnesyl pyrophospate (FPP) to zeaxanthin on the chromosome of Escherichia Coli. The enzyme presented on this page, CsADH2946 catalyzes the second reaction in the zeaxanthin to crocin pathway.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 195
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 602
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1119
Illegal SapI.rc site found at 227

References

1. Papandreou MA, Kanakis CD, Polissiou MG, Efthimiopoulos S, Cordopatis P, Margarity M, et al. Inhibitory Activity on Amyloid-β Aggregation and Antioxidant Properties of Crocus sativus Stigmas Extract and Its Crocin Constituents. J Agric Food Chem. 2006 Nov 1;54(23):8762–8.

2. Chen L, Qi Y, Yang X. Neuroprotective effects of crocin against oxidative stress induced by ischemia/reperfusion injury in rat retina. Ophthalmic Res. 2015;54(3):157–68.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2423001 short</partinfo>
-This part codes for aldehyde dehydrogenase 2946, which catalyses the conversion of crocetin dialdehyde into crocetin, using NAD+ as a cofactor, in Crocus sativus. It contains a constitutive promoter, as well as a His tag for easy purification. A version of this biobrick containing an inducible promoter is BBa_K2423007.
+This part codes for aldehyde dehydrogenase 2946, which catalyses the conversion of crocetin dialdehyde into crocetin, using NAD+ as a cofactor, in ''Crocus sativus''. It contains a constitutive promoter, as well as a His tag for easy purification. A version of this biobrick containing an inducible promoter is BBa_K2423007.
@@ Line 23: / Line 23: @@
 . Chen L, Qi Y, Yang X. Neuroprotective effects of crocin against oxidative stress induced by ischemia/reperfusion injury in rat retina. Ophthalmic Res. 2015;54(3):157–68.
-. Gómez-Gómez L, Parra-Vega V, Rivas-Sendra A, Seguí-Simarro JM, Molina RV, Pallotti C, et al. Unraveling Massive Crocins Transport and Accumulation through Proteome and Microscopy Tools during the Development of Saffron Stigma. Int J Mol Sci [Internet]. 2017 Jan 1;18(1). Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297711/
-. Lang BS, Gorren ACF, Oberdorfer G, Wenzl MV, Furdui CM, Poole LB, et al. Vascular Bioactivation of Nitroglycerin by Aldehyde Dehydrogenase-2. J Biol Chem. 2012 Nov 2;287(45):38124–34.