Difference between revisions of "Part:BBa K2381012"

Revision as of 03:11, 2 November 2017 (view source) MinTT (Talk | contribs) ← Older edit		Revision as of 03:14, 2 November 2017 (view source) MinTT (Talk | contribs) Newer edit →
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	The targeting sequence of this part is acquired from reference paper.		The targeting sequence of this part is acquired from reference paper.
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−	<img src="https://static.igem.org/mediawiki/parts/d/d2/T--HZAU-China--gRNA_function.png" width="800px"/>	+	<img src="https://static.igem.org/mediawiki/parts/d/d2/T--HZAU-China--gRNA_function.png" width="750px"/>
	</br>		</br>
	<b>BBa_K2381024</b>, is proved by OD measurement after cotransformed with plasmid containing dCas9. Its effect is even better than rapamycin, a well-used chemical molecule for replication inhibition.		<b>BBa_K2381024</b>, is proved by OD measurement after cotransformed with plasmid containing dCas9. Its effect is even better than rapamycin, a well-used chemical molecule for replication inhibition.
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	</br>		</br>
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−	<img src="https://static.igem.org/mediawiki/parts/2/26/T--HZAU-China--gRNA_qPCR.png" width="800px"/>	+	<img src="https://static.igem.org/mediawiki/parts/2/26/T--HZAU-China--gRNA_qPCR.png" width="750px"/>
	</br>		</br>
	Further investigation is conducted by qPCR and flow cytometric.		Further investigation is conducted by qPCR and flow cytometric.
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	</br>		</br>
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−	<img src="https://static.igem.org/mediawiki/parts/f/f1/T--HZAU-China--light_gRNA.png" width="800px"/>	+	<img src="https://static.igem.org/mediawiki/parts/f/f1/T--HZAU-China--light_gRNA.png" width="750px"/>
	</br>		</br>
	Its function after light-induced promoter, <b>BBa_K2381023</b>, is proved by OD measurement.		Its function after light-induced promoter, <b>BBa_K2381023</b>, is proved by OD measurement.

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Revision as of 03:14, 2 November 2017

3'-OriC gRNA

It’s a gRNA sequence containing 20bp targeting at oriC and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.
The targeting sequence of this part is acquired from reference paper.

BBa_K2381024, is proved by OD measurement after cotransformed with plasmid containing dCas9. Its effect is even better than rapamycin, a well-used chemical molecule for replication inhibition.

Further investigation is conducted by qPCR and flow cytometric.



Its function after light-induced promoter, BBa_K2381023, is proved by OD measurement.

References

Wiktor, J., Lesterlin, C., Sherratt, D. J., & Dekker, C. (2016). CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res, 44(8), 3801-3810.

Sequence and Features