Difference between revisions of "Part:BBa I719005"
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|The production of GFP decreases to minimal levels after 2 hours and tends towards nil after 4 hours.
 
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<html><hr><h3 style="color:ltblue;"><a href="http://2010.igem.org/Team:Warsaw/Stage1/PromMeas">Team Warsaw 2010's measurement</a></h3></html>
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Absolute promoter strength: 41,8pg RNA/minute/ug substrate DNA

Revision as of 22:53, 16 October 2010

T7 Promoter

Constitutive promoter derived from the T7 bacteriophage. Allows high expression of proteins only when the T7 polymerase is present. This part is identical to the part BBa_R0085 which currently hasn't been built.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization in vitro

This part has been characterized for temperature sensitivity in the Cell-Free Chassis by iGEM Imperial 2007. For more detail, please check the testing [http://parts.mit.edu/igem07/index.php/Imperial/Wet_Lab/Protocols/Prot1.9 protocols]and [http://parts.mit.edu/igem07/index.php/Imperial/Wet_Lab/Results/Res1.9 results].

I719005 invitro.png
Parameter Value and Description
Optimum temperature The T7 promoter has a highest output at 25°C, with a one-fold increase in GFP molecules synthesized compared to 37°C. The T7 promoter also has a minimal amount of output at 4°C.
Expression Life-span The rate of GFP synthesis by the T7 promoter reaches a peak at around 30-60 minutes.
Peak Expression The production of GFP decreases to minimal levels after 2 hours and tends towards nil after 4 hours.

Team Warsaw 2010's measurement

Absolute promoter strength: 41,8pg RNA/minute/ug substrate DNA