Difference between revisions of "Part:BBa K2195000"

Revision as of 02:52, 2 November 2017

Dsup (Radiation Protection)

Dsup is a protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species Ramazzottius Varieornatus. Dsup was discovered to protect this tardigrade species from X-ray damage [1]. Since only X-ray damage was tested, we decided to test if Dsup can protect organisms from UV radiation as well since it was not theorized to be a repair mechanism.

Usage and Biology

We found that Dsup can protect DH5α E. coli from UV-B radiation that normally causes pyrimidine dimers in the DNA. Using our UV irradiation protocol, we found that E. coli cells with the Dsup gene had a lower death rate/higher survival rate than our control cells.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 240
Illegal BamHI site found at 694
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 02:49, 2 November 2017 (view source) Markcwang (Talk \| contribs) ← Older edit		Revision as of 02:52, 2 November 2017 (view source) Markcwang (Talk \| contribs) Newer edit →
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−	~~This protein~~ is a protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species <i>Ramazzottius Varieornatus</i>. It was ~~found~~ to ~~be a protein that protects tardigrades~~ from X-ray damage ~~and is currently believed to be a DNA binding protein~~. Since only X-ray damage was tested, we decided to test if Dsup can protect organisms from UV radiation as well since it was not theorized to be a repair mechanism.	+	Dsup is a protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species <i>Ramazzottius Varieornatus</i>. Dsup was discovered to protect this tardigrade species from X-ray damage [1]. Since only X-ray damage was tested, we decided to test if Dsup can protect organisms from UV radiation as well since it was not theorized to be a repair mechanism.