Difference between revisions of "Part:BBa K2255008"

Revision as of 07:45, 21 October 2020

p3_E.coli (Rfc25)

This part is sequence coding for the D1 and D2 of the protein 3 of M13. Those domains are required for M13 adsorption and entry.

This part was design with Freiburg (Rfc25) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.

The collection part use for the creation of phage-like particle, those part correspond to the domain D1 and D2 of the p3 protein. They allow the phage-like particle to target the specific bacterium. To test the specificity of our phage-like particle and to target other pathogenic bacterium we design a large scale of attachment protein. This part is the first one of M13 attachment protein collection.

All the parts of our collection :

Table showing the attachment proteins from various filamentous phages.

Edit by the 2020 iGEM Montpellier team

Protein p3 is located at the end of the phage, there are around 5 copies of the protein and they are the largest and most complex proteins of the coat ones.

P3 is composed of three domains, two of which constitute this part. Domain 3 is the fixation of the protein to the rest of the pahge while 1 and 2 allow M13 adsoption and entry in the host cell. Both are necessary but the target bacteria also needs a pilus in order to be infected. The infection process also involves the TolA complex of the bacteria. The two domains 1 and 2 are able to bind with TolA-III but domain 1 has a better affinity than domain 2. However this remains true only if the bacteria has a pilus with which domain 2 interacts. In this case the adsorption is a success and the viral DNA is imported in the bacteria. In the other way, domain 2 binds with TolA-III and the infection does not work.

The Structure of D1 and D2 have been determined by different techniques thanks to which the previous mechanism has be elucidated. But the D3 structure remains unsolved.

Source: Sachdev S. Sidhu, Engineering M13 for phage display, Biomolecular engineering, September 2001 Lutz Riechmann and Philipp Holliger, The C-Terminal Domain of TolA Is theCoreceptor for Filamentous Phage Infectionof E. coli, Cell, 25th July 1997 John Armstrong, Richard N. Perham and John E. Walker, Domain structure of bacteriophage fd adsorption protein, FEBS letters, November 1981

Usage and Biology

This biobrick will be use to engineer phage-like particles targetting E. coli, this will enable us to test the fonctionality of our particles. Check out our [http://2017.igem.org/Team:Aix-Marseille/M13_Design design page] to know more about their design.

Sequence and Features

1. ↑ Smeal, S. W., Schmitt, M. A., Pereira, R. R., Prasad, A. & Fisk, J. D. Simulation of the M13 life cycle I: Assembly of a genetically-structured deterministic chemical kinetic simulation. Virology 500, 259–274 (2017).
2. ↑ Piekarowicz, A. et al. Neisseria gonorrhoeae Filamentous Phage NgoΦ6 Is Capable of Infecting a Variety of Gram-Negative Bacteria. J Virol 88, 1002–1010 (2014).
3. ↑ Luiten, R. G., Schoenmakers, J. G. & Konings, R. N. The major coat protein gene of the filamentous Pseudomonas aeruginosa phage Pf3: absence of an N-terminal leader signal sequence. Nucleic Acids Res 11, 8073–8085 (1983).
4. ↑ ^{4.0 4.1} T, K. et al. Genomic characterization of the filamentous integrative bacteriophages {phi}RSS1 and {phi}RSM1, which infect Ralstonia solanacearum., Genomic Characterization of the Filamentous Integrative Bacteriophages φRSS1 and φRSM1, Which Infect Ralstonia solanacearum. J Bacteriol 189, 189, 5792, 5792–5802 (2007).
5. ↑ Heilpern, A. J. & Waldor, M. K. pIIICTX, a predicted CTXphi minor coat protein, can expand the host range of coliphage fd to include Vibrio cholerae. J. Bacteriol. 185, 1037–1044 (2003).
6. ↑ Ikema, M. & Honma, Y. A novel filamentous phage, fs-2, of Vibrio cholerae O139. Microbiology 144, 1901–1906 (1998).
7. ↑ Campos, J. et al. VGJφ, a Novel Filamentous Phage of Vibrio cholerae, Integrates into the Same Chromosomal Site as CTXφ. J. Bacteriol. 185, 5685–5696 (2003).
8. ↑ Tseng, Y.-H., Lo, M.-C., Lin, K.-C., Pan, C.-C. & Chang, R.-Y. Characterization of filamentous bacteriophage ΦLf from Xanthomonas campestris pv. campestris. Journal of general virology 71, 1881–1884 (1990).
9. ↑ Chen, J. & Civerolo, E. L. Morphological evidence for phages in Xylella fastidiosa. Virology Journal 5, 75 (2008).
10. ↑ Ahmad, A. A., Askora, A., Kawasaki, T., Fujie, M. & Yamada, T. The filamentous phage XacF1 causes loss of virulence in Xanthomonas axonopodis pv. citri, the causative agent of citrus canker disease. Front. Microbiol. 5, (2014).