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===Usage and Biology===
 
===Usage and Biology===
 
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We found that Dsup can protect DH5α E. coli from UV-B radiation that normally causes pyrimidine dimers in the DNA. Using our UV irradiation protocol, we found that E. coli cells with the Dsup gene had a lower death rate/higher survival rate than our control cells.
  
 
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Revision as of 02:49, 2 November 2017


Dsup (Radiation Protection)

This protein is a protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species Ramazzottius Varieornatus. It was found to be a protein that protects tardigrades from X-ray damage and is currently believed to be a DNA binding protein. Since only X-ray damage was tested, we decided to test if Dsup can protect organisms from UV radiation as well since it was not theorized to be a repair mechanism.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 240
    Illegal BamHI site found at 694
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]