Difference between revisions of "Part:BBa K2381001"
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If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker. | If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker. | ||
</p> | </p> | ||
| + | <h3>Reference</h3> | ||
| + | <p>Kawano, F., Suzuki, H., Furuya, A., & Sato, M. (2015). Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun, 6, 6256. doi:10.1038/ncomms7256</p> | ||
</html> | </html> | ||
Latest revision as of 01:58, 2 November 2017
nMagHigh
nMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52D/M55G. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52R/M55R. They can heterodimerize under blue light irritation (470 nm, 3 mW/cm2). Their light sensing molecule is flavin adenine dinucleotide (FAD) which is normal in nearly all organisms.
This part is nMag-High, whose binding efficiency is much higher than the origin nMag. It's function is proved by parts
If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker.
Reference
Kawano, F., Suzuki, H., Furuya, A., & Sato, M. (2015). Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun, 6, 6256. doi:10.1038/ncomms7256
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 403