Difference between revisions of "Part:BBa K2457003:Design"
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===Design Notes=== | ===Design Notes=== | ||
This sequence is, 1130 bp of lenght, based on Escherichia coli strain MG1655 (Zhao 2016). This RecA is E. coli codon optimized, by changing codons from EcoRI and NotI sites in the codifying sequence of RecA, without removing the phase gene. | This sequence is, 1130 bp of lenght, based on Escherichia coli strain MG1655 (Zhao 2016). This RecA is E. coli codon optimized, by changing codons from EcoRI and NotI sites in the codifying sequence of RecA, without removing the phase gene. | ||
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===References=== | ===References=== | ||
Latest revision as of 02:03, 2 November 2017
Standardized RecA coding sequence
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 508
Illegal AgeI site found at 1012 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence is, 1130 bp of lenght, based on Escherichia coli strain MG1655 (Zhao 2016). This RecA is E. coli codon optimized, by changing codons from EcoRI and NotI sites in the codifying sequence of RecA, without removing the phase gene.
References
Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland, and Donald L. Court. An efficient recombination system for chromosome engineering in Escherichia coli. PNAS 2000 97 (11) 5978-5983; May 16, 2000
GENETIC ENGINEERING USING HOMOLOGOUS RECOMBINATION1 Donald L. Court, James A. Sawitzke, and Lynn C. Thomason. Annu. Rev. Genet. 2002. 36:361–88
Dongdong Zhao, Shenli Yuan, Bin Xiong, Hongnian Sun, Lijun Ye, Jing Li, Xueli Zhang and Changhao Bi .Development of a fast and easy method for Escherichia coli genome editing with CRISPR/ Cas9. 2016.