Difference between revisions of "Part:BBa K2365517"
Line 19: Line 19:
  
 
===Under testing===
 
===Under testing===
 +
[[File:JUB111.png|400px|center]]
 
[[File:JUB1.jpg|400px|center]]
 
[[File:JUB1.jpg|400px|center]]
  
 
To establish orthogonal regulators for use in yeast (Saccharomyces cerevisiae), we chose TFs from the plant Arabidopsis thaliana. We established a GFP green -based reporter system to test the effect of plant regulators in the yeast background.
 
To establish orthogonal regulators for use in yeast (Saccharomyces cerevisiae), we chose TFs from the plant Arabidopsis thaliana. We established a GFP green -based reporter system to test the effect of plant regulators in the yeast background.

Revision as of 01:21, 2 November 2017


JUB1 promoter

Arabidopsis NAC TFs JUNGBRUNNEN1 (JUB1)-------promoter (3*UAS)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 327
    Illegal BamHI site found at 432
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4


Under testing

JUB111.png
JUB1.jpg

To establish orthogonal regulators for use in yeast (Saccharomyces cerevisiae), we chose TFs from the plant Arabidopsis thaliana. We established a GFP green -based reporter system to test the effect of plant regulators in the yeast background.