Difference between revisions of "Part:BBa K2269017"
Line 7: Line 7:
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 +
 
Recombinase directionality factor (RDF) performance SILVER
 
Recombinase directionality factor (RDF) performance SILVER
  

Revision as of 00:34, 2 November 2017


Gp3 (PhiC31 RDF)

Streptomyces phage phiC31-encoded recombination directionality factor (RDF). Upon interaction with PhiC31 Integrase, it promotes site-specific recombination of attR and attL attachment sites to produce PhiC31 attP and attB sites. It has been optimized for N. Benthamiana chassis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]