Difference between revisions of "Part:BBa J364002:Experience"

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  [[File:T--Georgia State--BBa J364002 Georgia State 2017.png|600px|thumb|center|''Mean Fluorescence Intensity measured by flow cytometry of GFP expressed from 6-hour cultures of BBa_J364002 in multiple growth mediums.''
 
  [[File:T--Georgia State--BBa J364002 Georgia State 2017.png|600px|thumb|center|''Mean Fluorescence Intensity measured by flow cytometry of GFP expressed from 6-hour cultures of BBa_J364002 in multiple growth mediums.''
 
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=== 2019 Lethbridge iGEM Team ===
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<B> LB and minimal growth study for BBa_J364000, BBa_J364001 and BBa_J364002.</B>
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<p> In order to inform iGEM teams, we have compared these three parts in order to determine their growth and effectiveness in different conditions with the <i> E. coli </i> host. Each was test in LB and M9 minimal media to determine which part has the most resilience. Data was represented in colony forming units by using the microsphere calibration protocol from the 2018 measurement study. This will aid iGEM teams that will work in stress conditions for their projects. </p>
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<B> <p> please see our [https://2019.igem.org/wiki/images/c/cd/T--Lethbridge--characterization_data_Lethbridge_2019.pdf final report]to gain information on our media recipes, protocol and results.</p> </B>

Revision as of 00:04, 2 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J364002

User Reviews

UNIQf74d67ac7b7c91b1-partinfo-00000000-QINU UNIQf74d67ac7b7c91b1-partinfo-00000001-QINU


2017 Georgia State iGEM Team

Georgia State Growth Medium Study

Mean Fluorescence Intensity measured by flow cytometry of GFP expressed from 6-hour cultures of BBa_J364002 in multiple growth mediums.

2019 Lethbridge iGEM Team

LB and minimal growth study for BBa_J364000, BBa_J364001 and BBa_J364002.

In order to inform iGEM teams, we have compared these three parts in order to determine their growth and effectiveness in different conditions with the E. coli host. Each was test in LB and M9 minimal media to determine which part has the most resilience. Data was represented in colony forming units by using the microsphere calibration protocol from the 2018 measurement study. This will aid iGEM teams that will work in stress conditions for their projects.

please see our final reportto gain information on our media recipes, protocol and results.