Difference between revisions of "Part:BBa K2381012"
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It’s a gRNA sequence containing 20bp targeting at <i>oriC</i> and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.<br/> | It’s a gRNA sequence containing 20bp targeting at <i>oriC</i> and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.<br/> | ||
| − | The targeting sequence of this part is acquired from xxxx. Its function after J23119 promoter, <partinfo>BBa_K2381020</partinfo>, is proved by OD measurement after cotransformed with plasmid containing dCas9.<img src ="https://static.igem.org/mediawiki/parts/d/d2/T--HZAU-China--gRNA_function.png"> We can observe tight repression on cell growth. Further investigation is conducted by qPCR and flow cytometric.<img src ="https://static.igem.org/mediawiki/parts/2/26/T--HZAU-China--gRNA_qPCR.png"> | + | The targeting sequence of this part is acquired from xxxx. Its function after J23119 promoter, <partinfo>BBa_K2381020</partinfo>, is proved by OD measurement after cotransformed with plasmid containing dCas9.<img src ="https://static.igem.org/mediawiki/parts/d/d2/T--HZAU-China--gRNA_function.png" width="200px"> We can observe tight repression on cell growth. Further investigation is conducted by qPCR and flow cytometric.<img src ="https://static.igem.org/mediawiki/parts/2/26/T--HZAU-China--gRNA_qPCR.png" width="200px"> |
| − | Its function after light-induced promoter, <partinfo>BBa_K2381020</partinfo>, is proved by OD measurement. <img src ="https://static.igem.org/mediawiki/parts/f/f1/T--HZAU-China--light_gRNA.png"> | + | Its function after light-induced promoter, <partinfo>BBa_K2381020</partinfo>, is proved by OD measurement. <img src ="https://static.igem.org/mediawiki/parts/f/f1/T--HZAU-China--light_gRNA.png" width="200px"> |
</p> | </p> | ||
<h3>References</h3> | <h3>References</h3> | ||
Revision as of 23:38, 1 November 2017
3'-OriC gRNA
It’s a gRNA sequence containing 20bp targeting at oriC and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.
The targeting sequence of this part is acquired from xxxx. Its function after J23119 promoter, BBa_K2381020, is proved by OD measurement after cotransformed with plasmid containing dCas9.<img src ="
" width="200px"> We can observe tight repression on cell growth. Further investigation is conducted by qPCR and flow cytometric.<img src ="
" width="200px">
Its function after light-induced promoter, BBa_K2381020, is proved by OD measurement. <img src ="
" width="200px">
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]