Difference between revisions of "Part:BBa K525998"

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Summary: We tried to improve this promoter sequence by adding a Lac operator and making it RFC 10 compatible, since the Lac operator from pET-29a(+) has an illegal XbaI restriction enzyme site .  
 
Summary: We tried to improve this promoter sequence by adding a Lac operator and making it RFC 10 compatible, since the Lac operator from pET-29a(+) has an illegal XbaI restriction enzyme site .  
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The BBa_K525998 doesn't contain Lac operator sequence, and we managed to insert one in it so it would work better with the presence of LacI protein, making its regulation function better. See more information in <partinfo>BBa_K2382003</partinfo>.
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The BBa_K525998 doesn't contain Lac operator sequence, and we managed to insert one in it so it would work better with the presence of LacI protein, making its regulation function better.
 
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Documentation:
 
Documentation:

Revision as of 23:27, 1 November 2017

Promoter T7 and RBS

T7 promoter and RBS. The T7 promoter does not work with the RNA polymerase from Escherichia coli but with the RNA polymerase from the T7 phage. To express BioBricks under the control of a T7 promoter, E. coli carrying a T7 polymerase gene have to be used (e.g. BL21(DE3) or KRX).

Used to express e.g. BBa_K525123 - look there for typical induction profiles.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Improvement of K525998 by ZJU-CHINA 2017 Teams

The ZJU-CHINA 2017 Teams fulfilled the improvement of K525998 by inducing some mutation in the sequence. A series of promoters with different expression strength are constructed. The result can be seen by clicking the link below.

<a href=https://parts.igem.org/Part:BBa_K2207024>BBa_K2207024</a>

<a href=https://parts.igem.org/Part:BBa_K2207025>BBa_K2207025</a>

<a href=https://parts.igem.org/Part:BBa_K2207026>BBa_K2207026</a>

<a href=https://parts.igem.org/Part:BBa_K2207027>BBa_K2207027</a>

<a href=https://parts.igem.org/Part:BBa_K2207028>BBa_K2207028</a>

<a href=https://parts.igem.org/Part:BBa_K2207029>BBa_K2207029</a>

<a href=https://parts.igem.org/Part:BBa_K2207030>BBa_K2207030</a>

Contribution

Part name:BBa_K2382003
Group: iGEM17_CSMU_NCHU_Taiwan 2017
Author: TING-YU LIN
Summary: We tried to improve this promoter sequence by adding a Lac operator and making it RFC 10 compatible, since the Lac operator from pET-29a(+) has an illegal XbaI restriction enzyme site . The BBa_K525998 doesn't contain Lac operator sequence, and we managed to insert one in it so it would work better with the presence of LacI protein, making its regulation function better.
Documentation: