Difference between revisions of "Part:BBa K2324012"
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This part contains the <i> fim operon </i>, minus the <i>fimH</i> under control of a constitutive promoter. The constituent genes code for structural proteins, such as FimA which makes up the majority of the main body of a pilus, and others which support the process of pilus biogenesis. When co-transformed with FimH constructs from our project, which have rhamnose-inducible promoters, modified type I pili may be produced. The part requires no induction, and therefore is able to continuously produce the large mass of protein encoded in the operon. | This part contains the <i> fim operon </i>, minus the <i>fimH</i> under control of a constitutive promoter. The constituent genes code for structural proteins, such as FimA which makes up the majority of the main body of a pilus, and others which support the process of pilus biogenesis. When co-transformed with FimH constructs from our project, which have rhamnose-inducible promoters, modified type I pili may be produced. The part requires no induction, and therefore is able to continuously produce the large mass of protein encoded in the operon. | ||
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Revision as of 23:30, 1 November 2017
pJ23100_FimOp
This part contains the fim operon , minus the fimH under control of a constitutive promoter. The constituent genes code for structural proteins, such as FimA which makes up the majority of the main body of a pilus, and others which support the process of pilus biogenesis. When co-transformed with FimH constructs from our project, which have rhamnose-inducible promoters, modified type I pili may be produced. The part requires no induction, and therefore is able to continuously produce the large mass of protein encoded in the operon.
Fim Operon expression
![](https://static.igem.org/mediawiki/2017/e/ee/T--Exeter--MGNS.jpeg)
![](https://static.igem.org/mediawiki/2017/9/90/T--Exeter--Top10NS.jpeg )
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2648
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 931
Illegal AgeI site found at 962 - 1000COMPATIBLE WITH RFC[1000]