Difference between revisions of "Part:BBa K2455003:Design"
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| − | + | The nona-arginine CPP-tags is of synthetic origin, and is inspired by viral transduction domains. | |
===References=== | ===References=== | ||
Revision as of 23:16, 1 November 2017
Cell-Penetrating USER Cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The biobrick was created by amplifying a modified version of the pET102 vector with the following set of primers, followed by blunt ligation:
Forward primer: CGTCGCCGTCGCCGT-GAATGCGTGCGATCGCG
Reverse primer: GCGACGGCGACGCAT-GCGTATGTATATCTCCTTCTTAAAG
For creation of a biobrick version the following set of primers was sub-sequently used:
Forward primer: ATCCGGAATTCGCGGCCGCTTCTAG-ATGCGTCGCCGTCGCCGTCGCCGTCGCCGTGAATGCGTGCGATC
Reverse primer: CCAATGCATTGGTTCTGCAGCGGCCGCTACTAGTATTATTA-ATGGTGATGGTGATGATGTAAG
Source
The nona-arginine CPP-tags is of synthetic origin, and is inspired by viral transduction domains.