Difference between revisions of "Part:BBa K2355001"
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We tested the AMC-substrate peptide against the venom from our three snakes of interest, <i>Bitis arietans</i>, <i>Bitis gabonica</i> and <i>Naja nigricollis</i>. We made measurements in five different timepoints, with five different venom concentrations. The background noise was deducted from all measurements. The experiment showed that this particular substrate is cleaved significantly by the two venoms from <i>Bitis arietans</i> and <i>Bitis gabonica</i>, but not by the venom of <i>Naja nigricollis</i> as seen in figures 2-4. Different concentrations of venom had great effect in the fluorescence intensity.
 
We tested the AMC-substrate peptide against the venom from our three snakes of interest, <i>Bitis arietans</i>, <i>Bitis gabonica</i> and <i>Naja nigricollis</i>. We made measurements in five different timepoints, with five different venom concentrations. The background noise was deducted from all measurements. The experiment showed that this particular substrate is cleaved significantly by the two venoms from <i>Bitis arietans</i> and <i>Bitis gabonica</i>, but not by the venom of <i>Naja nigricollis</i> as seen in figures 2-4. Different concentrations of venom had great effect in the fluorescence intensity.
  
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<p>
 
         https://static.igem.org/mediawiki/2017/b/bc/T--DTU-Denmark--BA_AMC_experiment.png
 
         https://static.igem.org/mediawiki/2017/b/bc/T--DTU-Denmark--BA_AMC_experiment.png
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</p>
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<p>
 
         Figure 1: ALK-AMC incubated with different concentrations of Bitis arietans venom.
 
         Figure 1: ALK-AMC incubated with different concentrations of Bitis arietans venom.
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</p>
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<p>
 
         https://static.igem.org/mediawiki/2017/a/a9/T--DTU-Denmark--BG_AMC_experiment.png
 
         https://static.igem.org/mediawiki/2017/a/a9/T--DTU-Denmark--BG_AMC_experiment.png
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</p>
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<p>
 
         Figure 2: ALK-AMC incubated with different concentrations of Bitis gabonica venom.
 
         Figure 2: ALK-AMC incubated with different concentrations of Bitis gabonica venom.
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</p>
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<p>
 
         https://static.igem.org/mediawiki/2017/2/2d/T--DTU-Denmark--NN_AMC_experiment.png
 
         https://static.igem.org/mediawiki/2017/2/2d/T--DTU-Denmark--NN_AMC_experiment.png
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</p>
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<p>
 
         Figure 3: ALK-AMC incubated with different concentrations of Naja nigricollis venom.
 
         Figure 3: ALK-AMC incubated with different concentrations of Naja nigricollis venom.
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</p>
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<p>
 
In conclusion we were able to detect a significant difference from the Bitis species and the <i>Naja nigricollis</i>.
 
In conclusion we were able to detect a significant difference from the Bitis species and the <i>Naja nigricollis</i>.
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</p>

Revision as of 23:02, 1 November 2017


Cleavable peptide linker for Bitis arietans and Bitis gabonica venom

Peptide linker that is cleaved by Bitis arietans and Bitis gabonica venom.

When translated, this linker should contain 5 x repeats of the ALK motive identified by our experiments to be cleavable by the venom of Bitis sp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results of experiments

We tested the AMC-substrate peptide against the venom from our three snakes of interest, Bitis arietans, Bitis gabonica and Naja nigricollis. We made measurements in five different timepoints, with five different venom concentrations. The background noise was deducted from all measurements. The experiment showed that this particular substrate is cleaved significantly by the two venoms from Bitis arietans and Bitis gabonica, but not by the venom of Naja nigricollis as seen in figures 2-4. Different concentrations of venom had great effect in the fluorescence intensity.

T--DTU-Denmark--BA_AMC_experiment.png

Figure 1: ALK-AMC incubated with different concentrations of Bitis arietans venom.

T--DTU-Denmark--BG_AMC_experiment.png

Figure 2: ALK-AMC incubated with different concentrations of Bitis gabonica venom.

T--DTU-Denmark--NN_AMC_experiment.png

Figure 3: ALK-AMC incubated with different concentrations of Naja nigricollis venom.

In conclusion we were able to detect a significant difference from the Bitis species and the Naja nigricollis.