Difference between revisions of "Part:BBa K2492004"
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[[File:T--SUSTech Shenzhen--tracking.mp4|800px|thumb|left| Control the C. elegans by the light: We used the optical fiber to form a light spot and the worm can follow the spot.]]
 
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<p ><B>Fig.7 Control the <i>C. elegans</i> by the light.</B> We used the optical fiber to form a light spot and the worm can follow the spot.</p>
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[[File:T--SUSTech Shenzhen--getup.gif|800px|thumb|left| The test of the CoChR using light with different wavelengths and intensity. The lights blue- and blue+ is from the projector whose wavelength is about 480 and the lights whose wavelengths are 395, 440, 470, 560 and 640 are from the LED of fluorescence microscope (Nikon eclipse Ti).]]
 
[[File:T--SUSTech Shenzhen--getup.gif|800px|thumb|left| The test of the CoChR using light with different wavelengths and intensity. The lights blue- and blue+ is from the projector whose wavelength is about 480 and the lights whose wavelengths are 395, 440, 470, 560 and 640 are from the LED of fluorescence microscope (Nikon eclipse Ti).]]

Revision as of 02:22, 2 November 2017

pODR-10: the promoter of coding odorant diacetyl receptor protein.

Part BBa_K2492004 contains odr-10. It is a section of genomic DNA acts as a promoter. In C.elegans, ODR-10 encodes a large family of seven transmembrane olfactory receptor genes[1]which is specifically expressed in neuron AWA and affect chemotaxis to the volatile diacety. POrd-10 is a section of genemoic DNA in front of Ord-10 genes promoting expression of Ord-10 genes[2].

AWA is a member of the olfactory neurons [2]. It responses to attractive volatile odorants. Since we expect to express the functional channelrhodopsins and indicator in specific neuron(AWA for instance), we use pODR-10 to construct plasmid.

If CoCHR is expressed in AWA, we are capable of activating it to manipulate the state of AWA. In our project, we integrated ord-10::Chrimson::GEM-GECO::mCherry transgene to C.elegans by microinjection. Thus, CoCHR (Part BBa_K2492001:CoCHR) promoted by ord-10 is expressed in AWA. We can enhance the attraction of C.elegans through the control of AWA.

[1]:Sengupta P., Chou J.H., Bargmann C.I.Cell 84:899-909(1996)

[2]:Emily R. et al. (1997). Reprogramming Chemotaxis Responses: Sensory Neurons Define Olfactory Preferences in C. elegans, Cell, Vol. 91, 161–169.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

File:T--SUSTech Shenzhen--tracking.mp4

Fig.7 Control the C. elegans by the light. We used the optical fiber to form a light spot and the worm can follow the spot.

The test of the CoChR using light with different wavelengths and intensity. The lights blue- and blue+ is from the projector whose wavelength is about 480 and the lights whose wavelengths are 395, 440, 470, 560 and 640 are from the LED of fluorescence microscope (Nikon eclipse Ti).