Difference between revisions of "Part:BBa K2365508"
| Line 18: | Line 18: | ||
<partinfo>BBa_K2365508 parameters</partinfo> | <partinfo>BBa_K2365508 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
| − | |||
===[[File:图片3.png|300px|center]]=== | ===[[File:图片3.png|300px|center]]=== | ||
The 579bp fragment was cloned under the GAL1 promoter, followed by CYC1 terminator. The pYES2 vector is designed for native expression of protein of interest in S. cerevisiae. It contains the URA3 gene for selection in yeast and 2µ origin for high-copy maintenance. After verifying the right constructs by restriction enzyme digestions and sequencing, the plasmids were transformed into S. cerevisiae SEY6210. | The 579bp fragment was cloned under the GAL1 promoter, followed by CYC1 terminator. The pYES2 vector is designed for native expression of protein of interest in S. cerevisiae. It contains the URA3 gene for selection in yeast and 2µ origin for high-copy maintenance. After verifying the right constructs by restriction enzyme digestions and sequencing, the plasmids were transformed into S. cerevisiae SEY6210. | ||
Revision as of 22:37, 1 November 2017
Bax induced part
This part is used to as the key part of our biosafety device. Coding sequence of Bax protein is linked to the Gal1 promotor. It could be inserted to your device as kill switch when your chassis fungal is yeast(in the most broadly yeast).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 900
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 70
- 1000COMPATIBLE WITH RFC[1000]
The 579bp fragment was cloned under the GAL1 promoter, followed by CYC1 terminator. The pYES2 vector is designed for native expression of protein of interest in S. cerevisiae. It contains the URA3 gene for selection in yeast and 2µ origin for high-copy maintenance. After verifying the right constructs by restriction enzyme digestions and sequencing, the plasmids were transformed into S. cerevisiae SEY6210.