Difference between revisions of "Part:BBa K2276008"
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===Design Notes=== | ===Design Notes=== | ||
We want to engineer a strain which can produce melatonin at daily rhythm.We employ repressilator system thus allowing the E.coli coding enzyme periodically. However, the cycle time is not 24h.We built a model to analyse the issue.According to the medeling result, a stronger RBS was needed.We turned to the bioinformatic methods to design a RBS predicted to show hign translation rate.This is the website on which we performed the design: https://salislab.net/software/doForwardRBS. We designed three, and BBa_K2276007 is one of them. | We want to engineer a strain which can produce melatonin at daily rhythm.We employ repressilator system thus allowing the E.coli coding enzyme periodically. However, the cycle time is not 24h.We built a model to analyse the issue.According to the medeling result, a stronger RBS was needed.We turned to the bioinformatic methods to design a RBS predicted to show hign translation rate.This is the website on which we performed the design: https://salislab.net/software/doForwardRBS. We designed three, and BBa_K2276007 is one of them. | ||
Revision as of 22:43, 1 November 2017
a composite of TetR repressible promoter、special designed RBS and the coding sequence of mRFP
The part was designed based on the Part:BBa_I13521,while the RBS binding site is modified. The RBS is special designed for strains whose last 9 nucleotides of the 16S rRNA is ACCTCCTTA.We want to generate a part which present a high translation rate, but this one did not meet our expectation.The promoter is TetR repressible promoter which is also used in the repressilator system. And the mRFP served as the reporter to help us measure the translation rate of the designed RBS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 634
Illegal AgeI site found at 746 - 1000COMPATIBLE WITH RFC[1000]