Difference between revisions of "Part:BBa K2398009"
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This part provide bacteriophage M13 geneIII with a relatively weak RBS, sd8[[#References|[1]]], flanked by two homology regions for the usage due to the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). Figure one gives a short overview of our standard. Our BioBricks from the registry can easily be used for the assembly of blasmid with the standard (Fig.: 2). | This part provide bacteriophage M13 geneIII with a relatively weak RBS, sd8[[#References|[1]]], flanked by two homology regions for the usage due to the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). Figure one gives a short overview of our standard. Our BioBricks from the registry can easily be used for the assembly of blasmid with the standard (Fig.: 2). | ||
Revision as of 21:01, 1 November 2017
sd8-geneVI from M13 bacteriophage
This part provide bacteriophage M13 geneIII with a relatively weak RBS, sd8[1], flanked by two homology regions for the usage due to the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). Figure one gives a short overview of our standard. Our BioBricks from the registry can easily be used for the assembly of blasmid with the standard (Fig.: 2).
![](/wiki/images/c/cf/T--Heidelberg--Team_Heidelberg_2017_RFC_hd-1.jpeg)
![](/wiki/images/c/c4/T--Heidelberg--Team_Heidelberg_2017_RFC_hd.jpeg)
This part was used in the context of a subproject of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/CRISPR).
Figure 3 gives a short overview on how the plasmid was used.
![](/wiki/images/3/30/T--Heidelberg--Team_Heidelberg_2017_pampace1.png)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal BglII site found at 455 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Rferences
[1] Ringquist, S.; Shinedling, S.; Barrick, D.; Green, L.; Binkley, J.; Stormo, G. D.; Gold, L. (1992): Translation initiation in Escherichia coli: sequences within the ribosome-binding site. In: Molecular microbiology 6 (9), S. 1219–1229.