Difference between revisions of "Part:BBa K2398013"
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[[File:T--Heidelberg--Team_Heidelberg_2017_RFC_hd.jpeg|thumb|center|Figure 2: Compatibility of our cloning stadard with the RFC10;Any AP building block can be cloned into RFC[10] standard by inserting BglII sites between the homology regions and the biobrick prefix or suffix, respectively. To use such a part for AP assembly, it has to be digested with BglII. The resulting fragment should be purified and can subsequently used for Gibson assembly with other parts. ]] | [[File:T--Heidelberg--Team_Heidelberg_2017_RFC_hd.jpeg|thumb|center|Figure 2: Compatibility of our cloning stadard with the RFC10;Any AP building block can be cloned into RFC[10] standard by inserting BglII sites between the homology regions and the biobrick prefix or suffix, respectively. To use such a part for AP assembly, it has to be digested with BglII. The resulting fragment should be purified and can subsequently used for Gibson assembly with other parts. ]] | ||
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+ | The part was used for plasmid construction in combination with other parts from the iGEM Team Heidelberg part collection (http://2017.igem.org/Team:Heidelberg/Parts). It was applied in divers subprojects that can be found here: http://2017.igem.org/Team:Heidelberg/CRISPR, http://2017.igem.org/Team:Heidelberg/Cytochrome_Engineering | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Latest revision as of 20:44, 1 November 2017
p15A origin with Ampicillin resistance for application in Phage-assisted continous evolution (PACE)
This part consists of a p15A promoter and a ampicillin expression cassette, flanked by two homology regions for the usage due to the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). Figure one gives a short overview of our standard. Our BioBricks from the registry can easily be used for the assembly of blasmid with the standard (Fig.: 2).
![](/wiki/images/c/cf/T--Heidelberg--Team_Heidelberg_2017_RFC_hd-1.jpeg)
![](/wiki/images/c/c4/T--Heidelberg--Team_Heidelberg_2017_RFC_hd.jpeg)
The part was used for plasmid construction in combination with other parts from the iGEM Team Heidelberg part collection (http://2017.igem.org/Team:Heidelberg/Parts). It was applied in divers subprojects that can be found here: http://2017.igem.org/Team:Heidelberg/CRISPR, http://2017.igem.org/Team:Heidelberg/Cytochrome_Engineering
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal BglII site found at 2160 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 185
Illegal AgeI site found at 509 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1214