Difference between revisions of "Part:BBa K2271003"

Revision as of 20:45, 1 November 2017

Pex11-Venus with galactose inducible promoter

This part contains the coding sequence for the peroxin Pex11p with the galactose inducible promoter ScGAL1 and the terminator ScADH1. It is codon-optimized for S. cerevisiae and contains the fluorescent protein Venus fused to the 3'-end of the coding sequence. This is used in order to visualize the peroxin in the cell.

Usage and Biology

Pex11 is a peroxin located in the inner surface of the peroxisomal membrane and is required for peroxisome biogenesis [1]. Erdmann and Blobel have shown that the deletion of the PEX11 gene in S. cerevisiae results in cells with fewer, larger peroxisomes, whereas overexpression results in cells with a higher quantity of smaller peroxisomes [2].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 534
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 150
1000
COMPATIBLE WITH RFC[1000]

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 This part contains the coding sequence for the peroxin Pex11p with the galactose inducible promoter ScGAL1 and the terminator ScADH1. It is codon-optimized for S. cerevisiae and contains the fluorescent protein Venus fused to the 3'-end of the coding sequence. This is used in order to visualize the peroxin in the cell.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Pex11 is a peroxin located in the inner surface of the peroxisomal membrane and is required for peroxisome biogenesis [1]. Erdmann and Blobel have shown that the deletion of the PEX11 gene in S. cerevisiae results in cells with fewer, larger peroxisomes, whereas overexpression results in cells with a higher quantity of smaller peroxisomes [2].