Difference between revisions of "Part:BBa K2398001:Design"
 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
Synthetic part. <br>
 
Synthetic part. <br>
−
This was designed for the use with the cloning standard of the iGEM Team Heidelberg 2017.
+
This was designed for the use with the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). It is meant for the usage as building block for accessory plasmids in the context of phage assisted continuous ecolution [[#References|[1]]] or related systems [[#References|[2]]].
 +
 
 +
The part is fully compatible with RFC10, as well as with Golden Gate assembly
  
  
 
===Source===
 
===Source===
  
−
Bacteriophage T7  
+
Bacteriophage T7, with modifications.
  
 
===References===
 
===References===
 +
 +
[1] Esvelt, Kevin M.; Carlson, Jacob C.; Liu, David R. (2011): A system for the continuous directed evolution of biomolecules. In: Nature 472 (7344), S. 499–503. DOI: 10.1038/nature09929.
 +
 +
[2]Brödel, Andreas K.; Jaramillo, Alfonso; Isalan, Mark (2016): Engineering orthogonal dual transcription factors for multi-input synthetic promoters. In: Nature communications 7, S. 13858. DOI: 10.1038/ncomms13858.

Latest revision as of 19:39, 1 November 2017


T7 Promotor for application in Phage-assisted continous evolution (PACE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BglII site found at 258
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Synthetic part.
This was designed for the use with the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). It is meant for the usage as building block for accessory plasmids in the context of phage assisted continuous ecolution [1] or related systems [2].

The part is fully compatible with RFC10, as well as with Golden Gate assembly


Source

Bacteriophage T7, with modifications.

References

[1] Esvelt, Kevin M.; Carlson, Jacob C.; Liu, David R. (2011): A system for the continuous directed evolution of biomolecules. In: Nature 472 (7344), S. 499–503. DOI: 10.1038/nature09929.

[2]Brödel, Andreas K.; Jaramillo, Alfonso; Isalan, Mark (2016): Engineering orthogonal dual transcription factors for multi-input synthetic promoters. In: Nature communications 7, S. 13858. DOI: 10.1038/ncomms13858.