Difference between revisions of "Part:BBa K2398000"

Revision as of 17:43, 1 November 2017

Cytochrome c for the synthesis of organosilicons

We present a codon-optimized version of the cytochrome c protein derived from Rhodotermus marinus. This protein is able to perform the conversion of organosilicons. It is a novel catalytic unit that allows utilization of silicon compounds [Arnold et al., 2016]. When in its mature form, it catalyzes a carbene insertion into silicon-hydrogen bonds.

Usage and Biology

The cytochrome c is used for the catalysis of silicon compounds

Characterisation

[[File:T--Heidelberg--GCAnilineCompound.png|thumb|center|Figure 1: Gas chromatogram for the reaction of educt (1) and (5) to the product (3). 11.7 minutes retention time, indicates product formation. Unconverted educts converge 6.9 and 7.2, 7.4 minutes]

Figure 2: Mass chromatogram shows the breakdown of the product (3) ethyl 2-((4-aminophenyl)dimethylsilyl)propanoate. The product itself corresponds to a mass of 251 dalton

Figure 3: Gas chromatogram for the reaction of educt (2) and (5) to the product (4). 9.2 minutes retention time indicates product formation.

Figure 4: Mass chromatogram shows the breakdown of the product (4) ethyl 2-(dimethyl(phenyl)silyl)propanoate. The product itself corresponds to a mass of 236 dalton

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 93
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 ===Characterisation===
-[[File:T--Heidelberg--GCAnilineCompound.png|thumb|center|Figure 1: Gas chromatogram for the reaction of educt (1) and (5) to the product (3). 11.7 minutes retention time, indicates product formation. Unconverted educts converge 6.9 and 7.2, 7.4 minutes|]
+[[File:T--Heidelberg--GCAnilineCompound.png|thumb|center|Figure 1: Gas chromatogram for the reaction of educt (1) and (5) to the product (3). 11.7 minutes retention time, indicates product formation. Unconverted educts converge 6.9 and 7.2, 7.4 minutes]
-[[File:T--Heidelberg--MSAnilineCompound.png|thumb|center|Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates]]
+[[File:T--Heidelberg--MSAnilineCompound.png|thumb|center|Figure 2: Mass chromatogram shows the breakdown of the product (3) <i>ethyl 2-((4-aminophenyl)dimethylsilyl)propanoate</i>. The product itself corresponds to a mass of 251 dalton]]
-[[File:T--Heidelberg--GCNonAninlineCompound.png|thumb|center|Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates]]
+[[File:T--Heidelberg--GCNonAninlineCompound.png|thumb|center|Figure 3: Gas chromatogram for the reaction of educt (2) and (5) to the product (4). 9.2 minutes retention time indicates product formation.]]
-[[File:T--Heidelberg--MSNonAnilineCompound.png|thumb|center|Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates]]
+[[File:T--Heidelberg--MSNonAnilineCompound.png|thumb|center|Figure 4: Mass chromatogram shows the breakdown of the product (4) <i>ethyl 2-(dimethyl(phenyl)silyl)propanoate</i>. The product itself corresponds to a mass of 236 dalton]]